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How to take colonized grain spawn to bulk substrate in tubs, including how to pack your substrate so you don't get side pins.

CHAPTERS
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0:00 Materials & setup
4:43 Spawn to substrate ratio
9:12 Mixing spawn & substrate in tubs
11:38 Packing edges to prevent side pins
14:51 Adding a pseudo casing layer
16:32 Incubation & temperature
17:50 5 day colonization check
19:27 Into fruiting conditions

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▶ Watch next — How to grow mushrooms in an all-in-one bag [FULL GUIDE]: How to grow mushrooms in an all-in-one bag...

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GEAR USED IN THIS VIDEO
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I've bought a lot of gear and returned plenty of it — this is the stuff that actually earned a spot in my grow room. If any of it's useful to you and you buy through these affiliate links, I get a tiny cut at no cost to you. It genuinely helps me keep making these videos, and I appreciate you more than I can say.

Spawn & substrate
Grain spawn (Booming Acres): Oat and Milo Grain Bag
Substrate (Twisted Tree Nursery): Dino Soil

Tubs, tools & sterility
6 qt shoe box tubs: https://amzn.to/4xFyMl8
Stainless steel digital scale: https://amzn.to/4yQxncr
Isopropyl alcohol (70%): https://amzn.to/4cWuxtR
Nitrile gloves: https://amzn.to/4y5K3Me
Alcohol prep pads: https://amzn.to/4cWuLBd
Micropore tape: https://amzn.to/4gvLQEt

Incubation
Heat mat: https://amzn.to/4gx42xy
Thermometer / hygrometer: https://amzn.to/4zLSWw9
Baker's racks: https://amzn.to/4jjS2B2

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GEAR I USE IN OTHER STAGES
(not shown in this video)
For agar work, liquid culture & harvesting — covered in my other videos.
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Agar & culture work
Agar plates (90mm): https://amzn.to/4xtyDSy
Magnetic stir plate: https://amzn.to/3UEtB70
Slant tubes: https://amzn.to/4wYqZi7
Stainless steel racks: https://amzn.to/4xiXvfE
Metal racks (agar work): https://amzn.to/4cxy5CC

Liquid culture
Syringes: https://amzn.to/4wEN70K
Lids with injection ports: https://amzn.to/45DwOGn
8 oz. mason jars: https://amzn.to/4xNtHrd

Sterilizing
23 QT Presto pressure cooker: https://amzn.to/4xNBnKe

Inoculation
Spore/culture syringe: https://amzn.to/4qWevWQ
Sterile needles: https://amzn.to/3SzMK9L

Harvest & drying
Convection dehydrator: https://amzn.to/4qSlPCS
Silica gel packets: https://amzn.to/3UQAulT

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Thanks for watching!